186003975WATERS现货，广泛通用，耐受性强！
基于亚乙基桥杂化颗粒BEH，pH 1-12，耐受性强，高盐或高温条件下柱寿命更长?
与硅胶C18柱相比，活性硅醇基残留显著减少，能够提供更为尖锐、对称的峰形?
通用性强，广泛适用于各种肽的分析与分离?
提供两种孔径，130?和300?，以适应个别项目的特定需求?
涵盖不同规格色谱柱，包括：纳米级毛细管柱、UPLC柱、HPLC分析柱、OBD制备柱，满足从纳米级分析到实验室级别纯化的各阶段所需。
使用细胞色素C胰蛋白酶解物标准品、在0.1%TFA流动相条件下，对色谱柱填料批次进行质量控制，确保对验证方法提供稳定*的柱间重现性。
186003975WATERS现货
BEH130 C18与BEH300 C18的柱耐受性考察(低pH条件下加速)
色谱柱会因为物理原因和化学原因而失效。在低pH条件下，柱寿命短的主要原因是键合相的水解，这会导致肽保留的显著改变。BEH技术色谱柱包含了沃特世专有的键合与封端技术，能够产生稳定的键合相。在低pH条件下稳定性测试中，BEH C18柱显示出极少的保留损失。
经过严格测试以确保填料批次的可重现性
每一批ACQUITY UPLC和XBridge BEH 130和BEH300 C18填料，都经过严格测试以确保持续*的色谱性能。每根色谱柱的产品分析证书，都报告了该批次色谱填料的物理、化学和色谱测试结果，包括使用牛细胞色素C的胰蛋白酶解物作为混标检测的分离结果。
为肽的纯化简化色谱柱选择
可以使用单一C18柱，分离广泛范围下的各种肽。在UPLC柱或HPLC分析柱上所得的条件与分离，可以很方便的放大到相应的OBD制备柱上用于制备纯化，不同粒径的填料化学选择性**。OBD技术(Optimum Bed Density，柱床密度)，沃特世通过这项技术，以确保获得较稳定的填充柱床，从而延长制备柱的柱寿命(物理稳定性)。
肽分离技术制备柱选择指南
Prep OBD柱，5μm和10μm 130A 和 300A
| 内径 (mm) | 长度 (mm) | 单个肽μmol量 | 单个肽重量 (mg) | 典型流速 (mL/min) |
| 10 | 50 | 0.25–5 | 0.5–10 | 4.5–9 |
| 10 | 100 | 0.25–5 | 0.5–10 | 4.5–9 |
| 10 | 150 | 0.25–5 | 0.5–10 | 4.5–9 |
| 10 | 250 | 0.25–5 | 0.5–10 | 4.5–9 |
| 19 | 50 | 1–18 | 2.0–36 | 16–32 |
| 19 | 100 | 1–18 | 2.0–36 | 16–32 |
| 19 | 150 | 1–18 | 2.0–36 | 16–32 |
| 19 | 250 | 1–18 | 2.0–36 | 16–32 |
Prep OBD 柱，10 μm 130A 和 300A
| 内径 (mm) | 长度 (mm) | 单个肽μmol量 | 单个肽重量 (mg) | 典型流速 (mL/min) |
| 30 | 50 | 2.5–25 | 5–100 | 40–80 |
| 30 | 100 | 2.5–25 | 5–100 | 40–80 |
| 30 | 150 | 2.5–25 | 5–100 | 40–80 |
| 30 | 250 | 2.5–25 | 5–100 | 40–80 |
ACQUITY UPLC Peptide BEH130 和BEH300 C18柱
| 产品名称 | 粒径 | 柱内径 | 柱长 | 部件编号 |
| ACQUITY UPLC Peptide BEH130 C18 | 1.7 μm | 2.1 mm | 50 mm | 186003554 |
| ACQUITY UPLC Peptide BEH130 C18 | 1.7 μm | 2.1 mm | 100 mm | 186003555 |
| ACQUITY UPLC Peptide BEH130 C18 | 1.7 μm | 2.1 mm | 150 mm | 186003556 |
| ACQUITY UPLC Peptide BEH130 C18 | 1.7 μm | 2.1 mm | 300 mm | 186005792 |
| ACQUITY UPLC Peptide BEH300 C18 | 1.7 μm | 2.1 mm | 50 mm | 186003685 |
| ACQUITY UPLC Peptide BEH300 C18 | 1.7 μm | 2.1 mm | 100 mm | 186003686 |
| ACQUITY UPLC Peptide BEH300 C18 | 1.7 μm | 2.1 mm | 150 mm | 186003687 |
| ACQUITY UPLC Peptide BEH300 C18 | 1.7 μm | 1.0 mm | 50 mm | 186005592 |
| ACQUITY UPLC Peptide BEH300 C18 | 1.7 μm | 1.0 mm | 100 mm | 186005593 |
| ACQUITY UPLC Peptide BEH300 C18 | 1.7 μm | 1.0 mm | 150 mm | 186005594 |
以下为VanGuard预柱，用于保护UPLC柱，提高柱寿命
| 产品名称 | 部件编号 |
| BEH300, C18, 1.7 μm, VanGuard 预柱, 2.1 x 5 mm, 3/pk | 186004629 |
| BEH130, C18, 1.7 μm VanGuard 预柱, 2.1 x 5 mm, 3/pk | 186003975 |
以下为方法验证包，3根/包，来自不同批次填料，用于检查项目方法对填料批次的耐用性
| UPLC柱产品名称 | 粒径 | 柱内径 | 柱长 | 部件编号 |
| ACQUITY Peptide UPLC BEH130 C18 | 1.7 μm | 2.1 mm | 100 mm | 186004896 |
| ACQUITY Peptide UPLC BEH300 C18 | 1.7 μm | 2.1 mm | 100 mm | 186004897 |
| ACQUITY Peptide UPLC BEH130 C18 | 1.7 μm | 2.1 mm | 150 mm | 186006517 |
| ACQUITY Peptide UPLC BEH300 C18 | 1.7 μm | 2.1 mm | 150 mm | 186006516 |
| HPLC柱产品名称 | 粒径 | 柱内径 | 柱长 | 部件编号 |
| XBridge Peptide BEH130 C18 | 3.5 μm | 4.6 mm | 100 mm | 186004904 |
| XBridge Peptide BEH130 C18 | 5 μm | 4.6 mm | 100 mm | 186005463 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 4.6 mm | 100 mm | 186004905 |
| XBridge Peptide BEH300 C18 | 5 μm | 4.6 mm | 100 mm | 186005464 |
XBridge Peptide BEH130和BEH300 C18 HPLC分析柱(部分产品节选)
| 产品名称 | 粒径 | 柱内径 | 柱长 | 部件编号 |
| XBridge Peptide BEH130 C18 | 3.5 μm | 2.1 mm | 50 mm | 186003563 |
| XBridge Peptide BEH130 C18 | 3.5 μm | 2.1 mm | 100 mm | 186003564 |
| XBridge Peptide BEH130 C18 | 3.5 μm | 2.1 mm | 150 mm | 186003565 |
| XBridge Peptide BEH130 C 18 | 3.5 μm | 2.1 mm | 250 mm | 186003566 |
| XBridge Peptide BEH130 C18 | 3.5 μm | 4.6 mm | 50 mm | 186003567 |
| XBridge Peptide BEH130 C18 | 3.5 μm | 4.6 mm | 100 mm | 186003568 |
| XBridge Peptide BEH130 C18 | 3.5 μm | 4.6 mm | 150 mm | 186003569 |
| XBridge Peptide BEH130 C18 | 3.5 μm | 4.6 mm | 250 mm | 186003570 |
| XBridge Peptide BEH130 C18 | 5 μm | 2.1 mm | 50 mm | 186003574 |
| XBridge Peptide BEH130 C 18 | 5 μm | 2.1 mm | 100 mm | 186003575 |
| XBridge Peptide BEH130 C18 | 5 μm | 2.1 mm | 150 mm | 186003576 |
| XBridge Peptide BEH130 C18 | 5 μm | 2.1 mm | 250 mm | 186003577 |
| XBridge Peptide BEH130 C18 | 5 μm | 4.6 mm | 50 mm | 186003578 |
| XBridge Peptide BEH130 C18 | 5 μm | 4.6 mm | 100 mm | 186003579 |
| XBridge Peptide BEH130 C18 | 5 μm | 4.6 mm | 150 mm | 186003580 |
| XBridge Peptide BEH130 C18 | 5 μm | 4.6 mm | 250 mm | 186003581 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 2.1 mm | 50 mm | 186003607 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 2.1 mm | 100 mm | 186003608 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 2.1 mm | 150 mm | 186003609 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 2.1 mm | 250 mm | 186003610 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 4.6 mm | 50 mm | 186003611 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 4.6 mm | 100 mm | 186003612 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 4.6 mm | 150 mm | 186003613 |
| XBridge Peptide BEH300 C18 | 3.5 μm | 4.6 mm | 250 mm | 186003614 |
| XBridge Peptide BEH300 C18 | 5 μm | 2.1 mm | 50 mm | 186003618 |
| XBridge Peptide BEH300 C18 | 5 μm | 2.1 mm | 100 mm | 186003619 |
| XBridge Peptide BEH300 C18 | 5 μm | 2.1 mm | 150 mm | 186003620 |
| XBridge Peptide BEH300 C18 | 5 μm | 2.1 mm | 250 mm | 186003621 |
| XBridge Peptide BEH300 C18 | 5 μm | 4.6 mm | 50 mm | 186003622 |
| XBridge Peptide BEH300 C18 | 5 μm | 4.6 mm | 100 mm | 186003623 |
| XBridge Peptide BEH300 C18 | 5 μm | 4.6 mm | 150 mm | 186003624 |
| XBridge Peptide BEH300 C18 | 5 μm | 4.6 mm | 250 mm | 186003625 |
